Bioinformatics Laboratory (생명정보학 실험실)
: Our laboratory is conducting research utilizing a variety of sequencing technologies on the Genome, Epigenome, Transcriptome, and more. We focus on performing experiments and analyses using techniques such as Genome sequencing, RNA-seq, ChIP-seq, ATAC-seq, Hi-C seq, bisulfite-seq, and also on developing new technologies.
Experimental Method Development
We have developed and published single nucleus methyl 3C sequencing (snm3C-seq), a single-cell multimodal-omics technique that allows for the simultaneous observation of more than two features from a single cell (Nature Methods 2019). This was selected as the Technology of the Year in 2019 and is currently being used in the Brain Initiative project to study the brain's epigenetic profile.
Analysis Tool Development
We are developing analysis methods for various sequencing techniques. Among them, we have particularly studied Structural Variants (SVs) in cancer cells using Hi-C sequencing, and the resultant changes in the three-dimensional structure of the genome and their impact on gene expression, which was published in 2022 (Nature 2022).
In addition, we have developed all basic analysis pipelines for snm3C, which we previously developed, from the alignment stage, and we continue to develop programs for basic analysis (Nature Methods 2019).
In addition, we have developed all basic analysis pipelines for snm3C, which we previously developed, from the alignment stage, and we continue to develop programs for basic analysis (Nature Methods 2019).
Dong-Sung Lee, Chongyuan Luo, Jingtian Zhou, Sahaana Chandran, Angeline Rivkin, Anna Bartlett, Joseph R. Nery, Conor Fitzpatrick, Carolyn O’Connor, Jesse R. Dixon & Joseph R. Ecker, " Simultaneous profiling of 3D genome structure and DNA methylation in single human cells”, Nature Methods (2019).
Zhichao Xu, Dong-Sung Lee, Sahaana Chandran, Victoria T. Le, Rosalind Bump, Jean Yasis, Sofia Dallarda, Samantha Marcotte, Benjamin Clock, Nicholas Haghani, Chae Yun Cho, Kadir C. Akdemir, Selene Tyndale, P. Andrew Futreal, Graham McVicker, Geoffrey M. Wahl & Jesse R. Dixon. Structural variants drive context-dependent oncogene activation in cancer. (Nature 2022)
Dong-Sung Lee, Jong-Yeon Shin, Peter D. Tonge, Mira C. Puri, Seungbok Lee, Hansoo Park, Won-Chul Lee, Samer M. I. Hussein, Thomas Bleazard, Ji-Young Yun, Jihye Kim, Mira Li, Nicole Cloonan, David Wood, Jennifer L. Clancy, Rowland Mosbergen, Jae-Hyuk Yi, Kap-Seok Yang, Hyungtae Kim, Hwanseok Rhee, Christine A. Wells, Thomas Preiss, Sean M. Grimmond, Ian M. Rogers, Andras Nagy & Jeong-Sun Seo, An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator. (Nature Communications 2014)
